Aye Myat Thwe

Address:

Dundee Dental School

Park Place

Dundee

DD1 4HR

Phone Number:

Email Address:

a.m.thwe@dundee.ac.uk

B.D.S, Mres oral cancer

Project Title: EGF and TGFα motogenic activities are mediated by EGF reception: Identification of signalling pathways involved in oral cancer

Supervisors: Dr Ian Ellis and Profesor Peter Mossey

Background and Aims: Cells need to migrate away from their microenvironment to enable the tumour to metastasise. The growth factors Epidermal Growth Factor (EGF) and Transforming Growth Factor α (TGFα) stimulate the migration of fibroblasts into 3D collagen gels. Experiments using a number of growth factors concluded that some stimulated Akt phosphorylation whilst others reduced the phosphorylation of this pathway, but all stimulated migration; suggesting that multiple pathways are important for cell motility. EGF-R upon activation by ligand is phosphorylated at a number of residues. Each phosphorylated residue in turn stimulates specific signalling pathways.  The aim of the project is to determine which of these pathways is important for cell motility.

Methodology: Boyden chamber assay will be utilised to investigate the effects of specific kinase inhibitors on the migration of cells in the presence of EGF. Direct methods such as phospho-specific Western blotting will study the effect of EGF on phosphorylation of EGF-R.